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> need a loop that's capable of operating without Earth support for a significant length of time

Agree. A moon base lets us test this out with real-world constraints as opposed to simulants.

We don’t know how to build ecological closed loops. But every failure mode of Biosphere 2’s was almost trivially solvable with expendable components. (The CO2 cascade being the simplest among them.)

> idea that the best place to get started on working out how to do these sorts of things is on the moon

It’s not. It’s the best next step. We’re doing a lot of pre-colonisation lab work already. And we will need to do more before establishing a moon base. The moon base is the interim goal—you don’t put astronauts in swimming pools without a plan for a Shuttle. And Biosphere 2 is a terrible swimming pool for anything we’d do on the Moon or Mars.



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